Cuantificacion de taxol y baccatin iii en callos de taxus baccata por cromatografia liquida de alta eficiencia (hplc)


Authors

  • Yaned M. Correa
  • Jaime Niño
  • Oscar M. Mosquera

Abstract

Callos de Taxus baccata fueron inducidos a partir de segmentos de agujas y tallos en el medio de cultivo TM5 suplementado con diferentes concentraciones de picloram en combinación con ácido giberélico (GA3) y kinetina. Los segmentos de tallo produjeron callos con tasas de crecimiento más altas que las obtenidas a partir de las agujas. Los mejores medios para la inducción de callos fueron TM5 suplementados con 1 o 6 mg/L de picloram en combinación con 0,1 mg/L de GA3 y 0,1 mg/L de kinetina respectivamente. El análisis por HPLC de los extractos metanólicos de los callos determinó que el rango de concentración de Taxol estuvo entre 1,90–8,75 mg/L y el de Baccatin III entre 4,55–18,90 mg/L.

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Published

2006-01-12

How to Cite

Correa, Y. M., Niño, J., & Mosquera, O. M. (2006). Cuantificacion de taxol y baccatin iii en callos de taxus baccata por cromatografia liquida de alta eficiencia (hplc). Scientia Et Technica, 3(32). Retrieved from https://ojs2.utp.edu.co/index.php/revistaciencia/article/view/6335

Issue

Vol. 3 No. 32 (2006)

Section

Ciencias Básicas

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