INDUCCIÓN DE LA EMBRIOGÉNESIS SOMÁTICA A PARTIR DE CORMOS DE Crinum x powellii “album” (Amaryllidaceae)
DOI:
https://doi.org/10.22517/23447214.8937Keywords:
ácido 2, 4-diclorofenoxiacético, ácido giberélico, 6- benzilaminopurina, callos, embriones somáticos, kinetina, reguladores de crecimientoAbstract
Se indujo la producción de embriones somáticos (ESs) a partir de callos friables
formados de los cormos internos de la parte basal de los bulbos de Crinum x
powellii “album” cultivados en el medio Murashige y Skoog, suplementado con
el ácido 2,4-diclorofenoxiacético (1 mg/L) y kinetina (1.0 a 1.5 mg/L). La
inducción de los ESs se optimizó al utilizar 0.4 mg/L de 2,4-D y 0.375 mg/L
de kinetina; los ESs proliferaron en el medio MS libre de hormonas y después
de su desecación, se transfirieron al medio MS con ácido giberélico para su
maduración; a pesar de las variaciones físicas, de las condiciones y de los fitoreguladores,
los ESs no evolucionaron a plántulas.
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